ICSI for Gender Selection and Chromosomal Analysis:

Embryos are tested through preimplantation genetic diagnosis (PGD) before being transferred to the uterus, with an accuracy of up to 98%. The steps involved in embryo testing for gender are as follows:

• Sample Collection: After completing the ICSI process, when the fertilized egg reaches the third day of development (consisting of 6-8 cells), a single cell is extracted from the embryo through a tiny incision in the embryo’s wall without causing any damage. If the testing is performed on the fifth day, the sample is taken from the embryo, and the embryos are then frozen.
• Cell Analysis: The extracted cell is analyzed using chromosome staining techniques to determine the embryo’s sex and to detect any chromosomal abnormalities.
• Embryo Selection: In the final step, embryos with chromosomal abnormalities, such as Down syndrome, or those of an undesired sex are excluded. The healthy embryos, which meet the desired gender and are free from chromosomal abnormalities, are then transferred to the uterus.
• Post-Transfer Procedures: After embryo transfer, the woman uses progesterone supplements and waits for two weeks to confirm pregnancy.

Chromosomal analysis of embryos can also be performed in cases of repeated implantation failure or recurrent miscarriage.